1. |
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Molecular biotechnology |
Recombinant DNA technology, Commercialization of molecular biotechnology products, Concerns |
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2. |
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Recombinant DNA technology |
Restriction endonucleases, Ligation |
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Plasmid cloning vectors |
Plasmid cloning vectors, Plasmid cloning vector pBR322, Other plasmid cloning vectors |
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3. |
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Plasmid cloning vectors |
Cosmids, Types of cloning vectors |
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Creating and screening a library |
Making a genomic library, Screening by DNA hybridization / immunological assay / protein activity |
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4. |
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Creating and screening a library |
Cloning DNA sequences that encode eukaryotic proteins |
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Chemical synthesis of DNA |
Chemical synthesis of DNA |
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5. |
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Chemical synthesis of DNA |
Uses of synthesized oligonucleatides |
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Polymerase Chain Reaction |
Polymerase Chain Reaction |
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6. |
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DNA sequencing techniques |
DNA sequencing techniques |
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7. |
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Sequencing and imaging |
Cyclic reversible termination |
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Sequencing and imaging |
Sequencing by ligation, Pyrosequencing |
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8. |
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Sequencing and imaging, Functional genomics |
Real-time sequencing, GenBank sequence depository |
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Functional genomics |
Functional genomics, DNA microarray |
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9. |
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Functional genomics |
Senal analysis of gene expression(SAGE) |
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Proteomics |
Protein expression profiling: 2D differential in-gel electrophoresis & ICAT, Protein microarrays |
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10. |
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Manipulation of gene expression |
Basic features of gene expression control, Promoter control |
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11. |
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Manipulation of gene expressionFusion Proteins |
regulatable promoters, pCP3 expression system, Sigma factor |
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Fusion proteins |
Use of fusion protein, Cleavage of fusion protein |
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12. |
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other factors attecting gene expression |
Increasing protein stability, Increasing secretion, Metabolic load |
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13. |
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Directed mutagenesis |
Oligonucleotide-directed mutagenesis with plasmid, PCR amplified oligonucieotide directed mutagenesis |
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14. |
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Examples of protein engineering |
protein engineering introduction, Adding disulfide bonds, Increasing enzymatic activity, Decreasing protease sensitivity, Increasing enzyme stability and specificity |
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