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Molecular biotechnology | Recombinant DNA technology, Commercialization of molecular biotechnology products, Concerns | ![]() |
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Recombinant DNA technology | Restriction endonucleases, Ligation | ![]() |
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Plasmid cloning vectors | Plasmid cloning vectors, Plasmid cloning vector pBR322, Other plasmid cloning vectors | ![]() |
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Plasmid cloning vectors | Cosmids, Types of cloning vectors | ![]() |
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Creating and screening a library | Making a genomic library, Screening by DNA hybridization / immunological assay / protein activity | ![]() |
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Creating and screening a library | Cloning DNA sequences that encode eukaryotic proteins | ![]() |
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Chemical synthesis of DNA | Chemical synthesis of DNA | ![]() |
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Chemical synthesis of DNA | Uses of synthesized oligonucleatides | ![]() |
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Polymerase Chain Reaction | Polymerase Chain Reaction | ![]() |
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DNA sequencing techniques | DNA sequencing techniques | ![]() |
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Sequencing and imaging | Cyclic reversible termination | ![]() |
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Sequencing and imaging | Sequencing by ligation, Pyrosequencing | ![]() |
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Sequencing and imaging, Functional genomics | Real-time sequencing, GenBank sequence depository | ![]() |
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Functional genomics | Functional genomics, DNA microarray | ![]() |
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Functional genomics | Senal analysis of gene expression(SAGE) | ![]() |
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Proteomics | Protein expression profiling: 2D differential in-gel electrophoresis & ICAT, Protein microarrays | ![]() |
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Manipulation of gene expression | Basic features of gene expression control, Promoter control | ![]() |
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Manipulation of gene expressionFusion Proteins | regulatable promoters, pCP3 expression system, Sigma factor | ![]() |
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Fusion proteins | Use of fusion protein, Cleavage of fusion protein | ![]() |
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other factors attecting gene expression | Increasing protein stability, Increasing secretion, Metabolic load | ![]() |
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Directed mutagenesis | Oligonucleotide-directed mutagenesis with plasmid, PCR amplified oligonucieotide directed mutagenesis | ![]() |
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Examples of protein engineering | protein engineering introduction, Adding disulfide bonds, Increasing enzymatic activity, Decreasing protease sensitivity, Increasing enzyme stability and specificity | ![]() |