1. | ![]() |
Introduction | Biocatalysis, green chemistry, why proteins need to be engineered | ![]() |
![]() |
Introduction ProSAR approach | Biocatalysis, green chemistry, why proteins need to be engineered | ![]() |
|
2. | ![]() |
Enzyme Stability | Measuring protein stability | ![]() |
![]() |
Engineer Stable Proteins | Strategies to rationally engineer more stable proteins | ![]() |
|
![]() |
Irreversible unfolding via MG | Strategies to rationally engineer more stable proteins | ![]() |
|
3. | ![]() |
Making enzymes faster | Measuring reaction rates (MM kinetics) / Strategies to increase reaction rates | ![]() |
4. | ![]() |
Measure Selectivity | Measuring enzyme selectivity | ![]() |
![]() |
Engineer Selectivity | Strategies to increase selectivity | ![]() |
|
5. | ![]() |
Computer Modeling Overview | Viewing protein structures/ Molecular mechanics approach to modeling | ![]() |
![]() |
Critical evaluation of computer model | Extending molecular mechanics to model protein stability & pH dependence / Modeling selectivity (conformations & molecular dynamics) / Modeling reactivity (QM and other approaches) | ![]() |
|
6. | ![]() |
Mutagenesis Single Substitution | Overview of directed evolution: screening, random mutagenesis / Mutagenesis using error prone PCR - methods, polymerase fidelity | ![]() |
![]() |
Better NNK | Saturation mutagenesis approaches | ![]() |
|
![]() |
Circular permuation | Saturation mutagenesis approaches | ![]() |
|
7. | ![]() |
Multiple Substitutions | Bioinformatics approaches / Stepwise combinations of substitutions /Adding multiple mutations simultaneously | ![]() |
8. | ![]() |
Recombination Methods | Recombination methods - limitations, homology | ![]() |
9. | ![]() |
Genomic Approaches | Other approaches to mutagenesis (e.g. circular permutations, neutral drift libraries) | ![]() |
10. | ![]() |
Screening 1 | Screening strategies | ![]() |
![]() |
Screening 2 | Screening strategies | ![]() |
|
![]() |
Selection | Selection strategies | ![]() |
|
11. | ![]() |
open topic 1 | Expression Vectors | ![]() |
12. | ![]() |
open topic 2 | Metabolic Engineer | ![]() |