1. | Intro of students: interactive learning | Basic framework and current status; how we get to the conclusion/progress— key approaches and unexpected; Cell wall structure and function; Wall composition and cell type | ||
Intro of students: interactive learning | ||||
Intro of students: interactive learning | ||||
2. | Membrane structure | Formation of bilayers: It is an automatic/spontaneous process. Dynamics of lipid bilayer Fluidity, melting point, and phase transition of lipid bilayers Membrane TransportStructure? The only “structure” concerns the tonoplast—vacuole membrane. Single bilayer of 10 nM thick and resembles plasma membrane. What are the differences between the two membranes? Molecular markers. Most of plant cells have Large central vacuole. Bean mesophyll cells With chloroplasts and The central vacuole |
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Membrane structure | ||||
Membrane structure | ||||
3. | Plastids and Genome Interactions | proplastids:most simple and least developed form of plastids. Found in least differentiated tissues such as meristem and reproductive cells. Very small (1 um diameter) and primitive suborganelle structure. | ||
4. | Journey of a protein molecule | What is translation? Where is translation occurs in plant cells? Protein synthesis in chloroplasts Posttranslational modification, folding, assembly, and death |
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Journey of a protein molecule | What is translation? Where is translation occurs in plant cells? Protein synthesis in chloroplasts Posttranslational modification, folding, assembly, and death |
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Journey of a protein molecule | What is translation? Where is translation occurs in plant cells? Protein synthesis in chloroplasts Posttranslational modification, folding, assembly, and death |
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5. | Protein targeting to organelles | The problem: One place to make protein but many destinations—how does a protein know where to go and how to reach their “work place”? Quality control of ER proteins Quality control: unfolded proteins will be recognized by carrier proteins and transported back into the cytosol for degradation by ubiquitin-dependent proteasome. |
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6. | From Cytosol to organelles---post-translational translocation | 1)The envelope: this is the barrier that separates N from cytosolic space. Inner and outer membrane: the outer membrane is connected with ER, perinuclear space connected with ER lumen. 2) Nuclear pores Very large protein complex of more than 100 different proteins forms a basket-like structure through the double membranes |
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From Cytosol to organelles---post-translational translocation | ||||
From Cytosol to organelles---post-translational translocation | ||||
7. | Signal Transduction I---To put the cell in a “social context” | how does a cell in a multicellular organism communicate with other cells? how does a plant (cell) respond to the constant bomardments of “stimuli” from both inside and outside world? | ||
Signal Transduction I---To put the cell in a “social context” | ||||
Signal Transduction I---To put the cell in a “social context” | ||||
8. | Plant response to environment | How do plants respond to light? Photomorphogenesis --The light-induced developmental and morphological changes. So light not only serves as energy source for photosynthesis but also as a signal for growth and development. How does plants respond to light signal? |
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Plant response to environment | ||||
Plant response to environment | ||||
9. | Plant hormone signaling | What is a plant hormone? How to determine if a molecule functions as a hormone or not? Several criteria:1) produced by plant and has profound effect on growth and development 2) transportable (from one site to another inside the plant) 3) functions at a low level (often micromolar or less) 4) level regulated tightly by biosynthesis and degradation. |